Journal: Biomedical Optics Express

Article Title: In-vivo mechanical characterization of coronary atherosclerotic plaques in living swine using intravascular laser speckle imaging

doi: 10.1364/BOE.418939

Figure Lengend Snippet: First generation prototype ILSI catheter. (a) The distal end of the catheter, incorporating the single-mode optical fiber (SM600), the GRIN lens, the polarizer and the rod mirror to illuminate the arterial wall (Scale bar: 1 mm). Arterial speckle patterns are collected through the slanted rod mirror, transmitted by a leached fiber optical bundle (SCHOTT, USA; OD 0.7mm; 4.5k fibers; partial core size 0.36), and captured by a high-speed CMOS triggerable camera (Mikrotron, Germany). (b) Custom-fabricated double lumen sheath, housing the ILSI catheter. The inner lumen accommodates the leached fiber bundle and the illumination fiber. The outer lumen incorporates a proximal occlusion balloon (maximum outer dia. 3 mm) and a flushing port to clear the blood from imaging FOV. Reprinted with permission (Ref. [21]).

Article Snippet: A high speed triggerable CMOS camera (MC 1310, 500 fps @ 1280 × 1024 full resolution, dynamic range ∼ 59 dB, Mikrotron, Germany) was incorporated to record laser speckle patterns, ed by the optical catheter, at high acquisition rates (1000 frames per second (fps), 288 × 288 pixel size, ∼59 dB dynamic range).

Techniques: Imaging

Journal: Biomedical Optics Express

Article Title: In-vivo mechanical characterization of coronary atherosclerotic plaques in living swine using intravascular laser speckle imaging

doi: 10.1364/BOE.418939

Figure Lengend Snippet: Human coronary to live swine heart xenograft. A median sternotomy was performed to expose the beating heart of the anesthetized swine. The human xenograft was sutured to the anterior wall of the swine heart to simulate physiological motion. An aorto-atrial conduit redirected the blood flow to the grafted coronary via the first inlet of a Y connector. The second inlet allowed for the entrance of the ILSI catheter. A Doppler flowmeter monitored the blood stream redirected through the graft towards the right atrium. Arrow shows direction of blood from aorta (AO) to right atrium (RA). The ILSI catheter was housed in a double lumen sheath. The portable console was comprised of the He-Ne laser source and the bulk optics such as mirrors, beam expander, and fiber coupler (FC) for directing the light into the SMF. The arterial speckle patterns, imaged by the distal optics were transmitted by the fiber bundle to the high speed, triggerable CMOS camera. To generate the trigger signal, the EKG and femoral artery pressure waveforms were fed to a custom-made amplifier module. The amplified signals were digitized and processed by a data acquisition card (NI USB 6251 DAQ) incorporated in the console. The trigger pulse train was fed to the frame grabber for initiating the acquisition in synchrony with the pressure signal (Ref. [21]).

Article Snippet: A high speed triggerable CMOS camera (MC 1310, 500 fps @ 1280 × 1024 full resolution, dynamic range ∼ 59 dB, Mikrotron, Germany) was incorporated to record laser speckle patterns, ed by the optical catheter, at high acquisition rates (1000 frames per second (fps), 288 × 288 pixel size, ∼59 dB dynamic range).

Techniques: Amplification